Dengue is an endemic arbovirus disease and approximately 3.9 billion people from more than 125 countries (accounting for 40% of world population) are at risk. The four serotypes of dengue (1-4) can cause either self-limiting febrile flu-like illness called dengue fever (DF) or life threatening dengue hemorrhagic fever (DHF) characterized by thrombocytopenia and hemorrhage and dengue shock syndrome (DSS) due to vascular leakage.
The immune response to viral infection involves a complexity of both innate and adaptive pathways at the cellular and molecular level. At present patients infected with DENV can only be treated by intravenous fluid support to prevent hypovolemia and hypotensive shock. This treatment is less effective in severe cases if the diagnosis is delayed. Identification of therapeutics with both antiviral and immunomodulatory activity may lower patient mortality and reduce the burden of DENV on society. This research proposal aims to investigate the host-virus interaction to develop supportive measures.
The main objective of the proposed work was to investigate the virus-host interaction and subsequent immune response for better understanding the pathogenesis of dengue virus infection. The following works will be carried out:
- Validation of selected microRNA and immune related genes by PCR array and biochemical analysis
- Correlation of immune response with clinical severity
- Identification of possible immune regulated pathways involving in the pathogenesis of dengue virus infection using bioinformatics approach.
Why is this project Important?
- More than half of the world populations are under risk of dengue virus infection
- The disease is more severe in children under age 5
- No vaccine or antiviral is available which increases the mortality and morbidity associated with the disease
- Currently the only available treatment is providing supportive measures
- Understanding the host-virus interaction may help in finding suitable treatment options.
- Better understanding of virus-host interaction
- Opportunities for designing diagnostic, prognostic markers as well as treatment option
Total Budget: £6500
PCR array: £3500
Consumables and chemicals: £2000
Biochemical analysis: £1000
I am Dr. S. Gowri Sankar, a growing young researcher in the field of immunology and microbiology. I have finished my Ph.D in dengue virus immunology. Currently I am working in elucidating the role of genes and miRNA in dengue virus infection, which will pave the way for reducing the mortality and morbidity. I have several research publications in the field of dengue viral immunology. My contributions in the other field of science includes study on insect physiology. Our team is a group of researchers who were interested in the public health research especially on research and control of vector-borne diseases. Our teams ultimate goal is to work towards reducing the mortality and morbidity associated with vector-borne diseases.
Dr. Alwin Prem Anand, is a research scholar in Neuroscience. He has completed his PhD in Neuroscience from Germany. His work focuses on developmental neuroscience. He has research interests in developmental neurobiology and neurovirology. He has worked on several interdisciplinary research and published in international peer-reviewed journals
To thank you for your support
Your help will keep the research going and will boost morale to the people in the laboratory and help to understand the fundamentals in dengue virus infection.
The results will be presented in national and international conferences and published in international peer-reviewed journals.
Pledge £20 – Thank you for your support! A pledge at this level will pay for consumables for each sample
Pledge £50 – Thank you for your support! A pledge at this level will pay for biochemical analysis of each sample
Pledge £100 – Thank you for your support! A pledge at this level will pay for each sample PCR array
Pledge £500 – Thank you for your support. I will keep you updated on my experiments and share the results with you. I will send you a PDF of any manuscript that arises from this project and the contributors will be acknowledged wherever applicable.
Pledge £1000 – Thank you for your support. I will keep you updated on my experiments and share the results with you. I will send you a PDF of any manuscript that arises from this project and the contributors will be acknowledged wherever applicable.
I humbly thank you for your contribution.
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